期刊
JAPANESE JOURNAL OF PHARMACOLOGY
卷 89, 期 3, 页码 309-315出版社
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jjp.89.309
关键词
7-OH-DPAT; c-fos; northern blot; cerebellum; dopamine D-3 receptor
The effects of a preferential dopamine D-3 receptor agonist 7-hydroxy-NN'-di-n-propyl-2-aminotetralin (7-OH-DPAT) on c-fos mRNA expression in the rat cerebellum were studied by Northern blot analysis. 7-OH-DPAT (0.003-10 mg/kg, s.c.) markedly increased c-fos mRNA expression in the cerebellum, while its effects in the striatum, nucleus accumbens, and frontal cortex were negligible. The effect of 7-OH-DPAT on cerebellar c-fos mRNA expression was dose-dependent and statistically significant at doses of 0.3 mg/kg or more. A preferential dopamine D-2 agonist, bromocriptine (0.01-3 mg/kg, s.c.), failed to increase c-fos mRNA expression in the cerebellum. The effect of 7-OH-DPAT was blocked by two dopamine D-2-type-receptor antagonists, haloperidol and perospirone, but not the D-1-type-receptor antagonist SCH23390. Furthermore, dopaminergic denervation by 6-hydroxydopamine did not inhibit but rather potentiated the 7-OH-DPAT-induced c-fos mRNA expression in the cerebellum. These findings suggest that 7-OH-DPAT increases c-fos mRNA expression in the rat cerebellum, probably through postsynaptic dopamine D-3 receptor activation.
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