期刊
FUNGAL GENETICS AND BIOLOGY
卷 36, 期 2, 页码 147-154出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1087-1845(02)00011-7
关键词
Heterobasidion annostan; mycovirus; transmission; dsRNA; mitochondrial markers; nuclear markers; basidiomycete; plant pathogen; horizontal transfer; vertical transfer
Transmission of dsRNA viruses between homo- and heterokaryotic mycelia paired on agar plates and into conidia has been studied in Heterobasidion annosum. Horizontal transmission of dsRNA occurred between both homo- and heterokaryotic isolates, as well as between isolates belonging to different intersterility groups. The proportions of vertical transmission into conidia were 3% and 55%, respectively, for the two isolates included in the study. RT-PCR of dsRNA and PCR-RFLP of mitochondrial markers were used to confirm transmission of dsRNA between the cytoplasms of different mycelia. The identity of nuclei and nuclear migration during experiments were verified using PCR-RFLP of several nuclear markers. (C) 2002 Elsevier Science (USA). All rights reserved.
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