期刊
JOURNAL OF MAGNETIC RESONANCE
卷 157, 期 1, 页码 119-123出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/jmre.2002.2569
关键词
NMR resonance assignment; residual dipolar couplings; x-ray structure; amino-acid specific chemical shifts
资金
- NIGMS NIH HHS [GM66041] Funding Source: Medline
In protein NMR the assignment of nuclear spin resonances is a prerequisite for all subsequent applications, such as studies of ligand binding, protein-DNA interactions, and dynamics. Resonance assignment is a time consuming step even when the 3D x-ray structure of the protein is available. A new strategy is presented to solve the inverse assignment problem, which is the determination of the NMR resonance assignment from a known 3D protein structure. The protocol employs NMR data in the form of residual dipolar couplings and chemical shifts, while it does not require any sequential NMR connectivity information. The assignment problem is mathematically formulated in terms of a weighted matching problem that can be computationally efficiently solved by a combinatorial optimization algorithm. The protocol is applied to ubiquitin using two or three residual dipolar couplings per amino acid measured in Pfl phage medium together with chemical shift information. The algorithm yields for more than 90% of the protein backbone resonances the correct assignment. (C) 2002 Elsevier Science (USA).
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