期刊
JOURNAL OF LIPID RESEARCH
卷 43, 期 7, 页码 1026-1034出版社
ELSEVIER
DOI: 10.1194/jlr.M100435-JLR200
关键词
apolipoprotein B editing; immunoaffinity chromatography; retinol; retinyl esters
Although editing of apolipoprotein (apo)B in the small intestine, yielding apoB48, is thought to be nearly complete in adult humans, small amounts of intestinal apoB-100 may also be produced. We have evaluated the fraction of unedited apoB secreted from the intestine postprandially in subjects with primary combined hyperlipidemia, a disorder in which secretion of apoB-100 into the blood is increased. Three hours after these subjects and healthy controls were fed a fat-rich meal containing retinol, the distribution of retinyl esters (RE) between plasma triglyceride-rich lipoprotein (TRL) fractions containing apoB-100 and apoB48 was measured under conditions minimizing transfer of RE between lipoprotein particles. The estimated maximal percentage of unedited intestinal apoB-100 (similar to3%) was not increased in subjects with primary combined hyperlipidemia, suggesting that reduced editing of intestinal mRNA does not contribute to the pathogenesis of this disorder. Postprandially, the triglyceride content of TRL containing apoB48 more than doubled, leading to a 20% increase in mean diameter, yet the surface concentration of phospholipids and soluble apolipo-proteins (apoE and total apoC) was unchanged. Furthermore, the surface concentrations of these components did not differ among TRL containing apoB48 and two smaller fractions of apoB-100 TRL with distinct immunoreactivities. These findings suggest that available surface area is a major determinant of the particle content of each of these surface components of TRL species of differing size and origin. Sources and properties of triglyceride-rich lipoproteins containing apoB48 and apoB-100 in postprandial blood plasma of patients with primary combined hyperlipidemia.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据