期刊
PLANT CELL
卷 14, 期 7, 页码 1605-1619出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.003210
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资金
- NIAID NIH HHS [AI37832, R01 AI043288, AI43288, R37 AI043288, R01 AI027832, R21 AI043288] Funding Source: Medline
A large set of endogenous small RNAs of predominantly 21 to 24 nucleotides was identified in Arabidopsis. These small RNAs resembled micro-RNAs from animals and were similar in size to small interfering RNAs; that accumulated during RNA silencing triggered by multiple types of inducers. Among the 125 sequences identified, the vast majority (90%) arose from intergenic regions, although small RNAs corresponding to predicted protein-coding genes, transposon-like sequences, and a structural RNA gene also were identified. Evidence consistent with the derivation of small RNAs of both polarities, and from highly base-paired precursors, was obtained through the identification and analysis of clusters of small RNA loci. The accumulation of specific small RNAs was regulated developmentally. We propose that Arabidopsis small RNAs; participate in a wide range of post-transcriptional and epigenetic events.
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