期刊
ATHEROSCLEROSIS
卷 225, 期 2, 页码 322-327出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.atherosclerosis.2012.09.031
关键词
In vivo kinetics; Stable isotopes; Lp(a) assembly; HMW apo(a) isoforms; LMW apo(a) isoforms
资金
- Austrian Science Fund [P12358-MED]
- Austrian National Bank [6721/4]
- Fonds zur Forderung der Forschung an den Universitatskliniken Innsbruck [52, 9331]
- Austrian Science Fund (FWF) [P12358] Funding Source: Austrian Science Fund (FWF)
Objective: Lipoprotein(a) [Lp(a)] consists of apolipoprotein B-100 (apoB-100) as part of an LDL-like particle and the covalently linked glycoprotein apolipoprotein(a) [apo(a)]. Detailed mechanisms of its biosynthesis, assembly, secretion and catabolism are still poorly understood. To address the Lp(a) assembly mechanism, we studied the in vivo kinetics of apo(a) and apoB-100 from Lp(a) and LDL apoB-100 in nine healthy probands using stable-isotope methodology. Methods: The level of isotope enrichment was used to calculate the fractional synthesis rate (FSR), production rate (PR) and retention time (RT) using SAAMII software and multicompartmental modeling. Results: We observed a similar mean PR for apo(a) (1.15 nmol/kg/d) and apoB-100 (1.31 nmol/kg/d) from Lp(a), which differed significantly from the PR for apoB-100 from LDL (32.6 nmol/kg/d). Accordingly, mean FSR and RT values for Lp(a)-apo(a) were similar to those of Lp(a)-apoB and different from those for LDL-apoB. Conclusion: Two different kinetic apoB pools within Lp(a) and LDL suggest intracellular Lp(a) assembly from apo(a) and newly synthesized LDL. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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