期刊
ATHEROSCLEROSIS
卷 222, 期 2, 页码 367-374出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.atherosclerosis.2012.03.014
关键词
Phospholipolyzed LDL; hGX-sPLA(2); Lipid mediators; Dendritic cells; Immune response; Inflammation; Atherosclerosis
资金
- Government Francais-DREIC Jerusalem
- Institut National de la Sante et de la Recherche Medicale (Inserm)
- Agence Nationale pour la Recherche sur le SIDA et les hepatites (ANRS)
Objective: Increased secreted phospholipase A(2) (sPLA(2)) activity has been documented in several inflammatory disorders. Among sPLA(2)s, the human group X (hGX)-sPLA(2) has the highest catalytic activity towards phosphatidylcholine (PC), the major phospholipid of cell membranes and blood lipoproteins. hGX-sPLA(2) has been detected in human atherosclerotic lesions, indicating that sPLA(2)s are an important link between lipids and inflammation, both involved in atherosclerosis. The presence of dendritic cells (DC), the most potent antigen presenting cells, in atherosclerotic lesions has raised the question about their role in disease progression. Methods and results: In this study, we show that hGX-sPLA(2)-treated LDL induces human monocyte-derived DC maturation, resulting in a characteristic mature DC phenotype and enhanced DC ability to activate IFN gamma secretion from T cells. hGX-sPLA(2) phospholipolysis of LDL produces high levels of lipid mediators, such as lysophosphatidylcholine (LPC) and free fatty acids (FFAs), which also modulate DC maturation. The major molecular species of LPC containing a palmitic or stearic acid esterified in the sn-1 position induce DC maturation, whereas the FFAs can positively or negatively modulate DC maturation depending on their nature. hGX-sPLA(2) added alone can also activate DC in vitro through the hydrolysis of the DC membrane phospholipids leading, however, to a different cytokine profile secretion pattern than the one observed with hGX-sPLA(2)-phospholipolysed LDL. Conclusion: hGX-sPLA(2) secreted in inflamed tissues can contribute to local DC maturation, resulting in pro-Th1 cells, through the production of various lipid mediators from hydrolysis of either LDL and/or cell plasma membrane. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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