期刊
ATHEROSCLEROSIS
卷 221, 期 1, 页码 76-83出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.atherosclerosis.2011.12.022
关键词
Atherosclerosis; Adrenal; Steroidogenesis; Lymphocyte; LDL receptor knockout mice; Adrenalectomy; Cholesterol
资金
- TIPharma [T2-110]
- Netherlands Heart Foundation [2007T039, 2008T070]
Long-term changes in the secretion of immunosuppressive adrenal-derived glucocorticoid hormones influence cardiovascular disease risk. Here we determined the consequences of changes in adrenal steroid metabolism for the development of atherosclerotic lesions in mice. Atherosclerosis-susceptible low-density-lipoprotein (LDL) receptor knockout mice were subjected to adrenalectomy (ADX) or a control (SHAM) operation and subsequently fed an atherogenic diet for 4 weeks. Atherogenic diet feeding raised plasma corticosterone levels in SHAM mice, but not adrenalectomized mice, resulting in an 83% lower (P < 0.01) corticosterone level in adrenalectomized mice. Adrenalectomy was associated with a respectively 22% and 29% lower plasma level of cholesterol and triglycerides. In contrast, white blood cell counts were increased 2-fold (P < 0.01) in adrenalectomized mice, which could be attributed to a significant 2.1- to 2.6-fold rise in lymphocyte (P < 0.05) and monocyte (P < 0.05) numbers. Probably as a result of the enhanced systemic inflammatory status, adrenalectomy was associated with a higher susceptibility for diet-induced atherosclerosis (321 +/- 18 x 10(3) mu m(2) for ADX vs 240 +/- 31 x 10(3) mu m(2) for SHAM; P < 0.05) not withstanding the lowered cholesterol levels. Restoring adrenocortical steroid secretion - but not adrenal medulla function - and the associated downstream glucocorticoid receptor signaling in adrenalectomized mice through adrenal transplantation induced a reversal of the adrenalectomy-associated rise in white blood cell numbers, plasma monocyte chemoattractant protein 1 (MCP-1) levels, and atherosclerotic lesion development (lesion size in transplanted mice: 258 +/- 34 x 10(3) mu m(2); P < 0.05 vs ADX). In conclusion, our studies show that adrenal-derived steroids protect against the development of initial atherosclerotic lesions in LDL receptor knockout mice. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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