期刊
LICHENOLOGIST
卷 34, 期 -, 页码 351-359出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1006/lich.2002.0395
关键词
axenic culture; cultured mycobionts; gyrophoric acid chemosyndrome; Lobaria spathulata
The study aimed to optimize culture conditions and nutrient requirements for the production of secondary metabolites by the cultured mycobiont Lobaria spathulata. This species proved to be an excellent model system for such studies, as the complete chemosyndrome found in the natural lichen thallus was repeatedly formed in the cultured mycobiont with differentiated, aerial mycelia. Nutrient media containing the disaccharide, sucrose, were found to favour both rapid growth and the production of typical lichen substances. Higher proportions of the secondary compounds were detected in the developing mycobiont than in mature lichen thalli. (C) 2002 The British Lichen Society. Published by Elsevier Science Ltd. All rights reserved.
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