期刊
AQUACULTURE RESEARCH
卷 33, 期 8, 页码 607-613出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2109.2002.00698.x
关键词
antibody response; environmental temperature; indirect ELISA; sea bass; water oxygenation
类别
The influence of water temperature and water oxygenation on the specific antibody response was evaluated by indirect ELISA in sea bass (Dicentrarchus labrax L.) immunized against human-gamma- globulins emulsified in Freund's complete adjuvant. A higher antibody response was observed in fish reared at 24 and 30 degreesC, than at 12 and 18 degreesC. For the fish group reared at 24 degreesC, the immunological observation was carried out until 300 days after immunization. At the end of this period, fish kept at 24 degreesC still showed immunological competence, although the antibody response began to decrease significantly from 120 days after the immunization. Fish reared under mild hyperoxia and normoxia conditions had higher antibody responses than fish reared under mild hypoxia conditions. Moreover, at the end of the experimental period of 56 days the antibody response of fish reared under hyperoxia conditions was significantly higher than the immune response detected in fish reared under normoxia and hypoxia conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据