期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 295, 期 2, 页码 255-260出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0006-291X(02)00654-X
关键词
ghrelin; growth hormone; diurnal rhythm; feeding; pseudo-feeding; sheep; time-resolved fluoro-immunoassay; conditioned emotional response
Ghrelin, a gastric-derived peptide, has recently been identified as an endogenous natural ligand for the growth hormone (GH) secretagogue receptor. However, secretory characteristics of ghrelin are still obscure in ruminants. To investigate the diurnal rhythm in ghrelin secretion and its relationship to GH secretion, plasma ghrelin and GH concentrations were determined in Suffolk rams fed with a roughage diet once daily (Experiment 1). Abrupt increases (P < 0.05) in plasma ghrelin occurred just before a meal-feeding compared with that at I It before feeding, then rapidly fell with a minimum during the feeding. A pulsatile surge (P < 0.01) in plasma GH concentrations, which seemed to follow a single surge in plasma ghrelin, was observed during the feeding. In Experiment 2, plasma ghrelin and GH were determined in sheep subjected to a pseudo-feeding of 2 h to determine whether feed ingestion itself influences ghrelin and GH secretions. Compared with those at 1 h before feeding, a tendency of increases (P < 0.1) in plasma ghrelin and significant increases (P < 0.05) in GH occurred just before and during a pseudo-feeding, respectively. Plasma ghrelin temporally declined within I It after the start of the pseudo-feeding, and increased again and maintained higher levels during the last period of the pseudo-feeding. These results suggest that the transient surge of ghrelin secretion just before a scheduled meal feeding would not be due to the ingestion of feed, and that a pulsatile increase in plasma GH during the actual- or pseudo-feeding could be induced by the transient ghrelin surge. (C) 2002 Elsevier Science (USA). All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据