期刊
ANALYTICA CHIMICA ACTA
卷 463, 期 2, 页码 229-238出版社
ELSEVIER
DOI: 10.1016/S0003-2670(02)00417-8
关键词
laccase; biosensor; environmental monitoring; paper mill effluents; remediatiom; ozonization
This paper describes the development of a new system for amperometric determination of phenolic compounds, and its application for monitoring these compounds in paper mill effluent. The method was based on a flow system, a dialysis sampler, and a laccase-based biosensor. The performance of this system was investigated with respect to pH, ionic strength, working potential, and flow-rate dependence. The biosensor showed an excellent long-term stability allowing measurements for over than 3 months. The sensitivity of laccase-based biosensor was tested for phenol, p-chlorophenol, guaiacol and chloroguaiacol; the detector presented selective measurements of micromolar concentration of these compounds. The integration of a dialysis membrane sampling in the system protected the biosensor surface from fouling and gave independence of sample conditions that commonly influence the biosensor performance. These favorable characteristics allowed its application for direct measurements in complex media with no sample pretreatment. This ability was continued employing this system in a continuous analysis of phenolic compounds during the remediation of paper mill effluent by ozonization process. (C) 2002 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据