期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 91, 期 1, 页码 230-236出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0162-0134(02)00369-0
关键词
dibutyltin(IV); porphyrin; anti-tumor activity; DNA
The water-insoluble and water-soluble organotin(IV)porphinate complexes based on the tris-(4-pyridinyl)porphyrin and tris(N-methyl-4-pyridiniumyl)porphyrin moieties were synthesized and characterized by elemental analysis, H-1 NMR, IR and electrospray ionization mass spectra. The in vitro activity of the compounds against P388 leukemia and A-549 was determined. The results show that the anti-tumor activities of organotin(IV)porphinate is related to the water solubility of the compounds and the central ion in the porphyrin ring. The interaction between the water-soluble dibutyltin(IV) porphinate (7 and 10) complexes and DNA has been investigated. The result shows that compounds 7 and 10 cause DNA hypochromism measured by A,,,,, a slight increase in the viscosity of the DNA, and an increase in the melting point of DNA by 2.9 and 1.6 degreesC, respectively at DNA(base) /Drug(por) ratios of 60. The binding constants to DNA were 1.35+/-0.16x10(7) M-1 (7) and 1.45+/-0.12x10(6) M-1 (10) determined using EB competition method based on the porphyrin concentration, which is 20 and five times greater than that of precursor porphyrins [5-p,o-(carboxy)methoxyphenyl-10,15,20-tris(N-methyl-4-pyridiniumyl)] porphyrin (p,o-tMPyPac) to DNA. Electrophoresis test shows that the compounds cannot cleave the DNA. According to the electrophoresis test result and all the above results, the cytotoxic activity against P388 and A-549 tumor cells appears not to come from the cleavage of DNA caused by the compounds but from the high affinity of compounds to DNA. (C) 2002 Elsevier Science Inc. All rights reserved.
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