Effect of wash water temperature and chlorination on phenolic metabolism and browning of stored iceberg lettuce photosynthetic vascular tissues

Cut tissues from distinct anatomical locations in iceberg lettuce (Lactuca sativa L.) were subjected to washing in cold (4 degreesC) and warm (47 degreesC) water with or without chlorine to assess their propensity to discoloration during storage. Total protein (Bradford method) and phenolic (TPH; Folin-Ciocalteu method) contents and polyphenol oxidase (PPO; spectrophotometric method using catechol as a substrate), peroxidase (POD; guaiacol substrate), and phenylalanine ammonia-lyase (PAL; phenyl-alanine substrate) activities Were determined in photosynthetic and tissue from outer and inner leaves. Unprocessed photosynthetic and inner leaf tissues had significantly higher (P < 0.05) levels of protein and TPH and PPO and POD activities than, vascular and outer leaf tissues. PAL activities (on a fresh weight basis) were similar in all tissues. Changes in browning (light reflectance measurement) and phenolic. metabolism in all, four tissue types were observed during aerobic storage at 5 degreesC over 10 days.. PAL-activity increased in all tissues after 1-2 days of storage and then gradually decreased. POD activity also increased steadily for the storage duration. Protein content and PPO activity remained constant. Edge browning (measured with a Minolta Chroma Meter) and TPH increased in all tissues, especially in outer vascular tissue. Cut photosynthetic and vascular tissues washed at 4 and 47 degreesC with and without 1.00 mug mL(-1) chlorine for 3 min were analyzed during 7 days in storage at 5 degreesC. Enzyme activities and accumulation of phenolics in all tissues washed at 47 degreesC were significantly (P < 0.05) lower compared to controls or tissues washed at 4 degreesC. Chlorine had no additional effect at 47 degreesC but significantly (P < 0.05) reduced browning and accumulation of phenolics in lettuce washed at 4 degreesC. These results showed that inherent, differences between tissues affect phenolic metabolism and browning in stored, fresh-cut lettuce.