期刊
MECHANISMS OF DEVELOPMENT
卷 116, 期 1-2, 页码 117-127出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(02)00152-1
关键词
Dictyostelium; non-metazoan eukaryote; beta-catenin; aardvark; adherens junctions; stalk formation; cellulose stalk tube integrity; reporter gene expression
Aardvark (Aar) is a Dictyostelium P-catenin homologue with both cytoskeletal and signal transduction roles during development. Here, we show that loss of aar causes a novel phenotype where multiple stalks appear during late development. Ectopic stalks are preceded by misexpression of the stalk marker ST-lacZ in the surrounding tissue. This process does not involve the kinase GSK-3. Mixing experiments show that ectopic ST-lacZ expression and stalk formation are cell non-autonomous. The protein-cellulose matrix surrounding the stalk of aar mutant fruiting bodies is defective, and damage to the stalk of wild-type fruiting bodies leads to ectopic ST-lacZ expression. We postulate that poor synthesis of the stalk tube matrix allows diffusion of a stalk cell-inducing factor into the surrounding tissue. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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