期刊
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 48, 期 2, 页码 70-76出版社
BLACKWELL MUNKSGAARD
DOI: 10.1034/j.1600-0897.2002.01071.x
关键词
cytokine; trophoblast; vascular endothelial growth factor
PROBLEM: The mechanism through which vascular endothelial growth factor (VEGF) regulation occurs at the feto-maternal interface is poorly understood. The aim of this study was to investigate the effects of various cytokines on VEGF expression and secretion by trophoblast cells. METHOD OF STUDY: We investigated the effects of cytokines on VEGF expression in human first trimester trophoblast cell line by analyzing VEGF messenger RNA (mRNA) by reverse transcription-polymerase chain reaction and VEGF protein secretion by enzyme linked immunosorbent assay. RESULTS: The trophoblast cells expressed VEGF mRNA constitutively and the main subtypes were identified as VEGF121 and VEGF165. When cultured in the presence of interferon (IFN)-gamma, interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha IL-2. or IL-10. VEGF mRNA expression was found to be significantly increased by IL-1beta, IFN-gamma and TNF-alpha but to be unaffected by IL-2 and IL-10. Moreover, VEGF secretion was most significantly increased by IFN-gamma treatment. CONCLUSION: These results suggest that IL-1beta. IFN-gamma and TNF-alpha may regulate the production of VEGF in early gestational trophoblasts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据