期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 33, 期 3, 页码 364-369出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0891-5849(02)00895-X
关键词
myoglobin; 5,5-dimethyl-1-pyrroline N-oxide (DMPO); antibodies; protein radical; western blot; free radicals
This study reports the detection of protein free radicals using the specific free radical reactivity of nitrone spin traps in conjunction with nitrone-antibody specificity. Polyclonal antibodies were developed that bind to protein adducts of the nitrone spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO). The antibodies were used to detect DMPO protein adducts produced on horse myoglobin resulting from self-peroxidation. Western blot analysis demonstrates that myoglobin forms the predominant radical-derived nitrone adduct in rat heart Supernatant. Published by Elsevier Science Inc.
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