1H and 13C NMR study of 2-hydroxyglutaric acid and its lactone

Determination of the level and absolute configuration of 2-hydroxyglutaric acid in a patient's urine is a method of diagnosing two metabolic diseases. Such a determination can be done with the aid of NMR spectroscopic methods. In this paper the careful interpretation of H-1 and C-13 NMR spectra of this metabolite and its lactone measured under conditions used in biomedical assays is reported. The H-1 chemical shifts and spin-spin coupling constants were derived using the total lineshape analysis method. Copyright (C) 2002 John Wiley Sons, Ltd.