期刊
JOURNAL OF BACTERIOLOGY
卷 184, 期 15, 页码 4288-4295出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.184.15.4288-4295.2002
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A comprehensive study of Bacillus subtilis gene expression patterns in response to amino acid availability was performed by means of proteomics and transcriptomics. The methods of two-dimensional protein gel electrophoresis and DNA macroarray technology were combined to analyze cells exponentially grown in minimal medium with and without 0.2% Casamino Acids (CAA). This approach revealed about 120 genes predominantly involved in amino acid biosynthesis, sporulation, and competence, which were downregulated in CAA-containing medium. Determination of sporulation frequencies confirmed the physiological relevance of the expression data.
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