期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 50, 期 8, 页码 1067-1080出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/002215540205000809
关键词
cryoultramicrotomy; immunogold; PC12 cells; hippocampal neurons; electron microscopy; flat-embedding
类别
Immunogold labeling of ultrathin cryosections provides a sensitive and quantitative method to localize proteins at the ultrastructural level. An obligatory step in the routine preparation of cryosections from cultured cells is the detachment of cells from their substrate and subsequent pelleting. This procedure precludes visualization of cells in their in situ orientation and hampers the study of polarized cells. Here we describe a method to sample cultured cells from a petri dish or coverslip by embedding them in a 12% gelatin slab. Subsequently, sections can be prepared in parallel or perpendicular to the plane of growth. Our method extends the cryosectioning technique to applications in studying polarized cells and correlative light-electron microscopy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据