期刊
BIOTECHNOLOGY LETTERS
卷 24, 期 15, 页码 1257-1260出版社
SPRINGER
DOI: 10.1023/A:1016205924504
关键词
alcalase; amino acid; enantiomer; enzymatic resolution; ionic liquid
A new ionic liquid, N-ethyl pyridinium trifluoroacetate, was used with a commercial protease to resolve N-acetyl amino acid esters in place of traditional organic solvents. Products with enantiomeric excess (ee) between 86-97% were obtained. These results show that with low concentration of this new ionic liquid, the enzymatic resolution can be increased considerably depending upon the substrate being used.
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