期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 775, 期 2, 页码 143-152出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1570-0232(02)00278-7
关键词
vanadate; vanadium-transferrin complex
The behaviour of free [V-48]vanadate and [V-48] vanadium-transferrin complex was investigated on five different anion-exchange columns (Mono Q 515 HR, Hitrap Q HP, Sepharose Q FF, Sepharose DEAE FF and Hitrap Q XL). The recovery of both V-compounds was quantitative. The peak shape and retention time of vanadate varied according to the type of column. The vanadium-transferrin complex also showed different elution patterns depending on the type of column. Especially in case of the Sepharose Q FF, Mono Q 5/5 HR and Hitrap Q XL columns the vanadium-transferrin binding was degraded during elution on the column. The results clearly prove that care should be taken as to the choice of column for speciation purposes of vanadium compounds in order to prevent various artefacts showing up in the chromatograms. A Hitrap Q HP column was used to fractionate different vanadium compounds in rat serum. (C) 2002 Elsevier Science B.V. All rights reserved.
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