期刊
JOURNAL OF BIOTECHNOLOGY
卷 97, 期 2, 页码 107-116出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1656(02)00013-5
关键词
Pichia stipitis; water-hyacinth; ethanol; xylose-fermentation; hemicellulose acid hydrolysate
Water-hyacinth (Eichhornia crassipes) hemicellulose acid hydrolysate has been utilized as a substrate for ethanol production using Pichia stipitis NRRL Y-7124. Hydrolysate fermentability was considerable improved by boiling, and overliming up to pH 10.0 with solid Ca(OH)(2) in combination with sodium sulfite. The percent total sugar utilized and ethanol yield (Y-p/s) for the untreated hydrolysate were 20.15 +/- 0.17% and 0.19 +/- 0.003 g(p) g(s)(-1), respectively, compared with 76.0 +/- 0.32% and 0.35 g(p) g(s)(-1), respectively for the treated material. The fermentation was very effective at an aeration rate of 0.02 v/v/m, temperature 30 +/- 0.2 degreesC and pH 6.0 +/- 0.2. However, the volumetric productivity (Q(p)) was still considerably less than observed in a simulated synthetic hydrolysate medium with a sugar composition similar to the hemicellulose acid hydrolysate. L-Arabinose was not fermented but assimilated. The presence of acetic acid in the hydrolysate decreased the ethanol yield and productivity considerably. (C) 2002 Elsevier Science B.V. All rights reserved.
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