期刊
CANCER LETTERS
卷 182, 期 1, 页码 11-18出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3835(02)00056-3
关键词
N-nitrosomorpholine; N-methyl-N '-nitro-N-nitrosoguanidine; vitamin C; vitamin E; cytotoxicity; genotoxicity
类别
Since N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of N-nitrosomorpholine (NMOR) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Caco-2 cells. Cytotoxicity was evaluated by the trypan blue exclusion technique in Caco-2 cells and by the plating efficiency assay in V79 cells. NMOR caused a dose-dependent decline of viable cells in both cell lines; MNNG induced a dose-dependent cytotoxic effect only in V79 cells. Pretreatment of cells with vitamin C and vitamin E significantly reduced the cytotoxicity of NMOR, however, both vitamins had not effect on cytotoxicity induced by MNNG. These results suggest that different N-nitroso compounds react differently with cellular macromolecules. Measurement of the level of NMOR-induced DNA strand breaks and alkali-labile sites in both cell types using the alkaline comet assay also indicates a protective effect of both vitamins against the genotoxic effects of NMOR. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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