期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 543, 期 1, 页码 35-48出版社
WILEY
DOI: 10.1113/jphysiol.2002.020172
关键词
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资金
- NIA NIH HHS [AG00919, AG00836, K01 AG000919] Funding Source: Medline
- NINDS NIH HHS [NS30884] Funding Source: Medline
Permeation of the endogenous cation Zn2+ through calcium-permeable AMPA/kainate receptorgated (Ca-A/K) channels might subserve pathological and/or physiological signalling roles. Voltage-damp recording was used to directly assess Zn2+ flux through these channels on cultured murine hippocampal neurones. Ca-A/K channels were present in large numbers only on a minority of neurones (Ca-A/K(+) neurones), many of which were GABAergic. The presence of these channels was assessed in whole-cell or outside-out patch recording as the degree of inward rectification of kainate-activated currents, quantified via a rectification index (RI = G(+40)/G(-60)), which ranged from <0.4 (strongly inwardly rectifying) to >2 (outwardly rectifying). The specificity of a low RI as an indication of robust Ca-A/K channel expression was verified by two other techniques, kainate-stimulated cobalt-uptake labelling, and fluorescence imaging of kainate-induced increases in intracellular Ca2+. In addition, the degree of inward rectification of kainate-activated currents correlated strongly with the positive shift of the reversal potential (V,,v) upon switching to a sodium-free, 10 mm Ca2+ buffer. With Zn2+ (3 mM) as the only permeant extracellular cation, kainate-induced inward currents were only observed in neurones that had previously been identified as Ca-A/K(+). A comparison between the V-rev observed with 3 mm Zn2+ and that observed with Ca2+ as the permeant cation revealed a P-Ca/P-Zn of similar to 1.8. Inward currents recorded in 3 mm Ca2+ were unaffected 21 by the addition of 0.3 mm Zn2+, while microfluorimetrically detected increases in the intracellular concentration of Zn2+ in Ca-A/K(+) neurones upon kainate exposure in the presence of 0.3 mM Zn2+ were only mildly attenuated by the addition of 1.8 mm Ca2+. These results provide direct evidence that Zn 21 can carry currents through Ca-A/K channels, and that there is little interference between Ca2+ and Zn2+ in permeating these channels.
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