期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1572, 期 1, 页码 143-148出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-4165(02)00303-3
关键词
intracellular pH; BCECF; rapid measurement methodology; ammonium chloride challenge; Chinese hamster ovary cell; insect cell
A rapid intracellular pH (pH(i)) measurement method based on initial rate of increase of fluorescence ratio of 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein upon dye addition to a cell suspension in growth medium is reported. A dye transport model that describes dye concentration and fluorescence values in intracellular and extracellular spaces provides the mathematical basis for the approach. Experimental results of ammonium chloride challenge response of the two suspension cells, Spodoptera frugiperda and Chinese hamster ovary (CHO) cells, successfully compared with results obtained using traditional perfusion method. Since the cell suspension does not require any preparation, measurement of pH(i) can be completed in about 1 min minimizing any potential errors due to dye leakage. (C) 2002 Elsevier Science B.V All rights reserved.
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