期刊
JOURNAL OF CELL BIOLOGY
卷 158, 期 4, 页码 625-637出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200204136
关键词
yeast plasmid segregation; chromatin immunoprecipitation; monohybrid assay; cohesin recruitment; nondegradable Mcd1p
类别
资金
- NIGMS NIH HHS [GM 45185] Funding Source: Medline
The yeast 2 micron plasmid achieves high fidelity segregation by coupling its partitioning pathway to that of the chromosomes. Mutations affecting distinct steps of chromosome segregation cause the plasmid to missegregate in tandem with the chromosomes. In the absence of the plasmid stability system, consisting of the Rep1 and Rep2 proteins and the STB DNA, plasmid and chromosome segregations are uncoupled. The Rep proteins, acting in concert, recruit the yeast cohesin complex to the STB locus. The periodicity of cohesin association and dissociation is nearly identical for the plasmid and the chromosomes. The timely disassembly of cohesin is a prerequisite for plasmid segregation. Cohesin-mediated pairing and unpairing likely provides a counting mechanism for evenly partitioning plasmids either in association with or independently of the chromosomes.
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