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Antibody-based fluorescence detection of kinase activity on a peptide array

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BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 12, 期 16, 页码 2085-2088

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PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0960-894X(02)00378-5

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Peptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. (C) 2002 Elsevier Science Ltd. All rights reserved.

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