期刊
ONCOGENE
卷 21, 期 37, 页码 5753-5757出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1205680
关键词
allele-specific; Ras; functional genomics; peptide aptamer
资金
- NIGMS NIH HHS [GM57959] Funding Source: Medline
One challenge facing biology is the elucidation of the function of the estimated 30000 human genes and their polymorphic variants. Reagents that affect the activity of specific genes will be useful in the dissection of cellular regulatory networks. Here, as a test case, we used a two-bait two-hybrid system to identify peptide aptamers that distinguish allelic forms of H-Ras. Some of these anti-Ras aptamters inhibit the interaction of oncogenic Ras with c-Raf1 in vitro, and abolish EGF-stimulated activation of c-Raf1 in vivo. These experiments show that the inactivation of protein function by peptide aptamers represents a viable approach to the understanding and control of signaling pathways and oncogenic missense alleles.
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