期刊
NEUROSCIENCE LETTERS
卷 329, 期 2, 页码 177-180出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/S0304-3940(02)00644-4
关键词
St. John's wort; reactive oxygen species; neuroblastoma cell line; apoptosis; caspase-3 activity
The medicinal plant Hypericum perforatum Linn, commonly known as St. John's wort, has been used as an antidepressant. To investigate whether St. John's wort possesses a protective effect against hydrogen peroxide (H2O2)-induced cytotoxicity in neuronal cells, 3-(4,5-dimethylthiazol-2-yi)-2,5-diphenyltetrazolium bromide assay, 4,6-diamidino-2-phenylindole staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, flow cytometry analysis, DNA fragmentation assay, and caspase-3 enzyme assay were performed on SK-N-MC human neuroblastoma cells. Cells treated with H2O2 exhibited several apoptotic features, while those pre-treated with St. John's wort prior to H2O2 exposure showed a decreased occurrence of apoptotic features. In addition, pre-treatment with St. John's wort inhibited H2O2-induced increase in caspase-3 enzyme activity. These results suggest that St. John's wort may exert a protective effect against H2O2-induced apoptosis in human neuroblastoma cells. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据