Detection by enzyme-linked immunosorbent assay of antibodies to West Nile virus in birds

We adapted an indirect immunoglobuin G enzyme-linked immunosorbent assay to facilitate studies of West Nile virus (WNV) and evaluated its application to taxonomically diverse avian species. Anti-WNV antibodies were detected in 23 bird species, including many exotic species, demonstrating its value in studies of WNV epizootiology.