期刊
MOLECULAR BIOLOGY
卷 36, 期 5, 页码 698-704出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1023/A:1020631615748
关键词
reporter gene; licB; lichenase; thermostable enzyme; prokaryotic and eukaryotic transformed cells
A new reporter system was developed to study the gene expression regulation in prokaryotic (Escherichia coli) and eukaryotic (Saccharomyces cerevisiae and mammalian) cells. The system was based on the modified bacterial lichenase gene (licBM2), which was shown to meet the requirements fora reporter. The gene product was active and did not undergo modification in heterologous hosts. Simple and sensitive methods were used to detect and to quantitate the lichenase activity. Inducible licBM2 expression was demonstrated with E. coli and yeast cells, allowing the system to be employed in dynamic studies.
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