期刊
FASEB JOURNAL
卷 16, 期 11, 页码 1398-1406出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.02-0225com
关键词
ECaC; CaT1; vitamin D; calcium reabsorption
Pseudovitamin D-deficiency rickets (PDDR) is an autosomal disease characterized by hyperparathyroidism, rickets, and undetectable levels of 1,25-dihydroxyvitaminD(3) (1,25(OH)(2)D-3). Mice in which the 1,25-hydroxyvitamin D-3-1alpha-hydroxylase (1alpha-OHase) gene was inactivated presented the same clinical phenotype as patients with PDDR and were used to study renal expression of the epithelial Ca2+ channel (ECaC1), the calbindins, Na+/Ca2+ exchanger (NCX1), and Ca2+-ATPase (PMCA1b). Serum Ca2+ (1.20+/-0.05 mM) and mRNA/protein expression of ECaC1 (41+/-3%), calbindin-D-28K (31+/-2%), calbindin-D-9K (58+/-7%), NCX1 (10+/-2%), PMCA1b (96+/-4%) were decreased in 1alpha-OHase(-/-) mice compared with 1alpha-OHase(+/-) littermates. Feeding these mice a Ca2+-enriched diet normalized serum Ca2+ levels and expression of Ca2+ proteins except for calbindin-D-9K expression. 1,25(OH)(2)D-3 repletion resulted in increased expression of Ca2+ transport proteins and normalization of serum Ca2+ levels. Localization of Ca2+ transport proteins was clearly polarized in which ECaC1 was localized along the apical membrane, calbindin-D-28K in the cytoplasm, and calbindin-D-9K along the apical and basolateral membranes, resulting in a comprehensive mechanism facilitating renal transcellular Ca2+ transport. This study demonstrated that high dietary Ca2+ intake is an important regulator of the renal Ca2+ transport proteins in 1,25(OH)(2)D-3-deficient status and thus contributes to the normalization of blood Ca2+ levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据