期刊
CLINICAL BIOCHEMISTRY
卷 35, 期 6, 页码 455-461出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0009-9120(02)00330-2
关键词
alkaline phosphatase; inducible nitric oxide synthase; lipopolysaccharide; surfactant-like particle; cell culture; interleukin 6
Intestinal alkaline phosphatase (AP), as a host defense factor, was first investigated in vivo using rats orally exposed to lipopolysaccharide (LPS). After the oral administration of LPS to rats, serum LPS content was increased within 2 hr and then decreased to 6 hr. In contrast, when L-phenylalanine (L-Phe), an inhibitor of intestinal-type AP isozymes, was simultaneously administered with LPS, serum LPS content significantly increased from 1 hr and the area under the concentration-time curve of serum LPS was augmented approximately 2-fold, suggesting that APs in the gastrointestinal tract reduced serum LPS content. In addition, LPS toxicity diminished by a treatment in vitro with intestinal APs, were recovered by the treatment in the co-presence of L-Phe. In the experiment using human aortic endothelial cells (HAECs), we observed that the cell viability decreased in a dose-dependent manner of LPS-exposure, and the LPS dose, exhibiting 50% viability of the cells, was 0.05 mug/ml. When the cells were exposed to LPS pretreated with 50 nIU/mI of intestinal AP at pH 10.0 and 8.0, the 50% viability was at 2.0 mug/ml of LPS. These results strongly suggest that the APs reduced the toxicity of LPS, as a host defense factor against LPS. (D 2002 The Canadian Society of Clinical Chemists. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据