期刊
JOURNAL OF VIROLOGICAL METHODS
卷 105, 期 2, 页码 343-348出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-0934(02)00121-0
关键词
Cowpea mosaic virus; agroinfection; plant virus vectors
To increase the efficiency of infections with Con;pea mosaic virus (CPMV)-based constructs, clones suitable for agroinfection were constructed. Full-length copies of RNA-1 and RNA-2 were inserted between the sequence of a Cauliflower mosaic virus (CaMV) 35S promoter and a nos terminator and were introduced into the Agrobacterium tumefaciens plasmid, pBINPLUS. Infiltration of leaves of either Nicotiana benthamiana or cowpea (Vigna unguiculata) with a bacterial suspension containing a mixture of the RNA-1- and RNA-2-based plasmids resulted in the plants developing typical CPMV symptoms. To confirm the utility of this approach for use with CPMV-based vectors, a GFP construct based on RNA-2 was adapted for agroinfection. Infiltration of N. benthamiana leaves with a mixture of Agrobacteria containing this construct and the RNA-1 plasmid resulted in high levels of GFP expression. The results demonstrate that agroinfection is a suitable method for the propagation of CPMV-based derivatives. (C) 2002 Elsevier Science B.V. All rights reserved.
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