期刊
LEUKEMIA
卷 16, 期 9, 页码 1818-1826出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.leu.2402632
关键词
acute myeloid leukemia; NOD/SCID mice; flow cytometry; cDNA microarrays; gene expression
资金
- NCI NIH HHS [P01CA70970] Funding Source: Medline
Most cases of human acute myelold leukemia (AML) engraft in irradiated non-obese diabetic/severe combined immuno-deficient (NOD/SCID) mice. Intravenous transfer of as few as 105 human AML cells resulted in engraftment. Cases with poor prognosis clinical features, including FLT3 mutations, tended to engraft efficiently. Nevertheless, AML cells obtained from patients at relapse did not engraft more efficiently than cells obtained from the same patients at initial diagnosis. One passage of human AML cells in NOD/SCID mice did not appear to select for increased virulence, as measured by serial transplantation efficiency. Finally, cDNA microarray analyses indicated that similar to95% of genes were expressed at similar levels in human AML cells immunopurified after growth in mice, as compared to cells assessed directly from patients. Thus, the growth of human AML cells in NOD/SCID mice could yield large numbers of human AML cells for direct experimental use and could also function as a renewable, potentially unlimited source of leukemia cells, via serial transplantation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据