期刊
PLANTA
卷 215, 期 5, 页码 708-715出版社
SPRINGER
DOI: 10.1007/s00425-002-0816-3
关键词
cyclophilin; nitrite; nitric oxide; peroxynitrite; 14-3-3 protein; tyrosine nitration
An antisense nitrite reductase (NiR, EC 1.7.7.1) tobacco (Nicotiana tabacum L.) transformant (clone 271) was used to gain insight into a possible correlation between nitrate reductase (NR, EC 1.6.6.1)-dependent nitrite accumulation and nitric oxide (NO) production, and to assess the regulation of signal transduction in response to stress conditions. Nitrite concentrations of clone 271 leaves were 10-fold, and NO emission rates were 100-fold higher than in wild type leaves. Increased protein tyrosine nitration in clone 271 suggests that high NO production resulted in increased peroxynitrite (ONOO-) formation. Tyrosine nitration was also observed in vitro by adding peroxynitrite to leaf extracts. As in mammalian cells, NO and derivatives also increased synthesis of proteins like 14-3-3 and cyclophilins, which are both involved in regulation of activity and stability of enzymes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据