期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 66, 期 9, 页码 1819-1828出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.66.1819
关键词
cytochrome P450; Petunia hybrida; lauric acid; linoleic acid; linolenic acid
In higher plants, long-chain fatty acid hydroperoxides are intermediates in the synthesis of a diverse group of bioactive compounds. We used the reverse trascriptase-polymerase chain reaction to isolate a gene responsible for the oxidization of fatty acids from Petunia hybrida. A P450 cDNA not isolated earlier, CYP92B1, contained an open reading frame predicted to encode a polypeptide consisting of 510 amino acid residues. The transcript of the cyp92B1 gene was expressed at a high level in the early stage of flower development. CYP92B1 cDNA was expressed in a yeast, Saccharomyces cerevisiae, and recombinant yeast microsomes containing CYP92B1, a hemoprotein, metabolized lauric acid, linoleic acid, and linolenic acid.
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