期刊
MOLECULAR ECOLOGY NOTES
卷 2, 期 3, 页码 370-372出版社
WILEY
DOI: 10.1046/j.1471-8286.2002.00182.x
关键词
DNA bulking; DNA pooling; gene frequency; genetic markers; microsatellitcs; SNPs
Pooling of DNA samples can significantly reduce the effort of population studies with DNA markers. I present a statistical model and numerical method for estimating gene frequency when pooled DNA is assayed for the presence/absence of alleles. Analytical and Monte-Carlo methods examined estimation variance and bias, and hence optimal pool size, under a triangular allele frequency distribution. For gene frequency of rarer alleles, the optimal number of pooled individuals is approximately the inverse of the gene frequency. For heterozygosity, the optimal pool is approximately half the allele number; this results in pools containing, on average, 60% of possible alleles.
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