期刊
MOLECULAR CELL
卷 10, 期 3, 页码 537-548出版社
CELL PRESS
DOI: 10.1016/S1097-2765(02)00651-2
关键词
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资金
- NIGMS NIH HHS [GM62862-01] Funding Source: Medline
In Drosophila, two features of small interfering RNA (siRNA) structure-5' phosphates and 3' hydroxyls are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5' phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3' hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms.
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