期刊
PLASMID
卷 48, 期 2, 页码 117-129出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0147-619X(02)00120-8
关键词
Corynebacterium glutamicum; plasmid genome; antibiotic resistance; class 1 integron; gene cassette; tetracycline efflux system; horizontal gene transfer
We determined the complete nucleotide sequence of the 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum LP-6 which encodes streptomycin, spectinomycin, and tetracycline resistance. The antibiotic resistance determinant of pTET3 comprises an intIl-like gene. which was truncated by the insertion sequence IS6100, and the novel aminoglycoside adenyltransferase gene cassette aadA9. The deduced AADA9 protein showed 61%, identity and 71% similarity to AADA6 of integron In51 from Pseudomonas aeruginosa. In addition. pTET3 carries the novel repressor-regulated tetracycline resistance determinant Tet 33 which revealed amino acid sequence homology to group I tetracycline efflux systems. The highest level of similarity was observed to the tetracycline efflux protein TetA(Z) from the C glutamicum plasmid pAGI with 65% identical and 77%, similar amino acids. Each antibiotic resistance region of pTET3 is flanked by identical copies of the widespread insertion sequence IS6100 initially identified in Mycobacterium fortuitum. Transposition assays with a cloned copy of IS6100 revealed that this element is transpositionally active in C glutamicum. These data suggest a central role of IS6100 in the evolutionary history of pTET3 by mediating the cointegrative assembly of resistance gene-carrying DNA segments. (C) 2002 Elsevier Science (USA). All rights reserved.
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