期刊
JOURNAL OF BACTERIOLOGY
卷 184, 期 18, 页码 4952-4961出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.184.18.4952-4961.2002
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资金
- NIAID NIH HHS [AI 12575, R01 AI012575] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007197, T32 GM 07197, T32 GM 07183, T32 GM007183] Funding Source: Medline
Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.
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