期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 40, 期 9, 页码 3449-3454出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.40.9.3449-3454.2002
关键词
-
类别
资金
- NCRR NIH HHS [M01RR00052-39-5(S1), M01 RR000052] Funding Source: Medline
We describe a novel adaptation of the TaqMan PCR assay which potentially allows for highly sensitive detection of any eubacterial species with simultaneous species identification. Our system relies on a unique multiprobe design in which a single set of highly conserved sequences encoded by the 16S rRNA gene serves as the primer pair and is used in combination with both an internal highly conserved sequence, the universal probe, and an internal variable region, the species-specific probe. A pre-PCR ultrafiltration step effectively decontaminates or removes background DNA. The TaqMan system described reliabAly detected 14 common bacterial species with a detection limit of 50 fg. Further, highly sensitive and specific pathogen detection was demonstrated with a prototype species-specific probe designed to detect Staphylococcus aureus. This assay has broad potential in the clinical arena for rapid and specific diagnosis of infectious diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据