期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1555, 期 1-3, 页码 111-115出版社
ELSEVIER
DOI: 10.1016/S0005-2728(02)00264-5
关键词
mass spectrometry; peptide mapping; membrane protein; matrix-assisted laser desorption/ionization; CNBr; trypsin
A limitation of the in-gel approaches for the generation of peptides of membrane proteins is the size and hydrophobicity of the fragments generated. For membrane proteins like the lactose transporter (LacS) of Streptococcus thermophilus, tryptic digestion or CNBr cleavage yields several hydrophobic fragments larger than 3.5 kDa. As a result, the sequence coverage of the membrane domain is low when the in-gel tryptic-digested or CNBr-cleaved fragments are analyzed by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS). The combination of tryptic digestion and subsequent CNBr cleavage on the same gel pieces containing LacS approximately doubled the coverage of the hydrophobic membrane domain compared to the individual cleavage methods, while the coverage of the soluble domain remained complete. The fragments formed are predominantly below m/z 2500, which allows accurate mass measurement. (C) 2002 Published by Elsevier Science B.V.
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