期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 50, 期 19, 页码 5343-5348出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf0203500
关键词
Terminalia catappa L.; supercritical CO2 extraction; squalene; antioxidant activity; GC-MS
Squalene was identified by gas chromatography- mass spectrometry and high-performance liquid chromatography (HPLC) spiking analyses in the supercritical CO2 extracts of freeze-dried abscisic leaves of Terminalia catappa L. When the freeze-dried abscisic, senescent, mature, and immature leaves and seeds were subjected to supercritical CO2 extraction at 40 degreesC and 3000 psi and HPLC quantitation, squalene contents were 12.29, 2.42, 1.75, 0.9, and 0% in the extracts and corresponding to 1499, 451, 210, 65, and 0 mug/g in the freeze-dried sample, respectively. When the extracts were applied for antioxidative characterization by supplementation in an iron/ascorbate system with linoleic acid and in a pork fat storage system for inhibition of conjugated diene hydroperoxide (CDHP) formation or in a free radical scavenging system with 1,1-diphenyl-2-picryl-hydrazyl (DPPH), the extracts of leaves exhibited potent antioxidative and DPPH scavenging activities and increased with an increase of leaf maturity. However, the seed extracts only exhibited potent inhibition of CDHP formation and very low DPPH scavenging activity.
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