期刊
CANCER LETTERS
卷 183, 期 2, 页码 163-168出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3835(02)00031-9
关键词
esculetin; retinoblastoma protein; cell cycle arrest; leukemia cell; cyclin; cyclin-dependent kinase
类别
Esculetin, a coumarin compound, was found to inhibit cell growth and cell cycle progression by inducing arrest of the G1 phase in HL-60 cells. To obtain information regarding cell cycle arrest induced by esculetin, we examined its effect on the regulating factors of the G1 phase in the leukemia HL-60 cells treated with esculetin by Western blotting. Our observations were: (1) a distinct increase in the level of hypophosphorylated retinoblastoma protein (pRb), and a reduction in the level of CDK4 after treatment with 100 muM of esculetin for 24 h; (2) a marked up-regulation of p27, and a down-regulation of cyclin D1 after treatment with 100 muM esculetin for 24 h. These results suggest that esculetin can inhibit the growth of human leukemia HL-60 cells by 61 phase cell cycle arrest as a result of inhibited pRb phosphorylation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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