期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 520, 期 1-2, 页码 103-111出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1383-5718(02)00168-7
关键词
Fenton reagent; hydroxyl radical; N-nitrosamine; nitric oxide
N-Nitrosodimethylamine (NDMA) in phosphate buffer was rapidly decomposed by Fenton reagent composed of H2O2, and Fe(11) ion. Electron spin resonance (ESR) studies using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) showed that characteristic four line 1:2:2:1 ESR signals due to the DMPO-OH adduct formed on treatment of DMPO with Fenton reagent disappeared in the presence of NDMA, and N-nitrosodiethylamine (NDEA), suggesting the interaction of the N-nitrosamines with Fenton reagent. Treatment of the N-nitrosamines with Fenton reagent generated nitric oxide (NO) as estimated by ESR technique using cysteine-Fe(II), and N-methyl-D-glucaminedithiocarbamate (MGD)-Fe(II) complexes. Characteristic 3, and single line signals due to 2 cysteine-Fe(II)-NO, and 2 cysteine-Fe(II)-2 NO complexes, respectively, and three line signals due to MGD-Fe(II)-NO were observed. Considerable amount of NO were liberated as determined by NO2-, the final oxidation product of NO formed by reaction with dissolved oxygen in the aqueous medium. Spontaneous release of a small amount of NO from the N-nitrosamines was observed only on incubation in neutral buffers. Above results indicate that the N-nitrosamines were decomposed accompanying concomitant release of NO on contact with reactive oxygen species. (C) 2002 Elsevier Science B.V. All rights reserved.
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