期刊
CARBOHYDRATE RESEARCH
卷 337, 期 17, 页码 1593-1598出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0008-6215(02)00186-6
关键词
cactus; Opuntia ficus-indica; Prickly pear seeds; 4-O-methyl-glucuronoxylan; H-1 and C-13 NMR spectroscopy
Xylans were isolated from the pericarp of prickly pear seeds of Opuntia ficus-indica (OFI) by alkaline extraction, fractionated by precipitation and purified. Six fractions were obtained and characterized by sugar analysis and NMR spectroscopy. They were assumed to be (4-O-methyl-D-glucurono)-D-xylans, with 4-O-alpha-D-glucopyranosyluronic acid groups linked at C-2 of a (1 --> 4)-beta-D-xylan. The sugar composition and the H-1 and C-13 NMR spectra showed that their chemical structures were very similar, but with different proportions of D-Xyl and 4-O-Me-D-GlcA. Our results showed that, on average, the water soluble xylans have one nonreducing terminal residue of 4-O-methyl-D-glucuronic acid for every 11 to 14 xylose units, whereas in the water non-soluble xylans, xylose units can varied from 18 to 65 residues for one nonreducing terminal residue of 4-O-methyl-D-glucuronic acid. (C) 2002 Elsevier Science Ltd. All rights reserved.
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