期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 79, 期 7, 页码 703-712出版社
WILEY
DOI: 10.1002/bit.10378
关键词
metabolome analysis; metabolomics; pathway structure; functional genomics; convex analysis; elementary flux modes; Saccharomyces cerevisiae
In the field of functional genomics increasing effort is being undertaken to analyze the function of orphan genes using metabolome data. Improved analytical equipment allows screening simultaneously for a high number of metabolites. Such metabolite profiles are analyzed using multivariate data analysis techniques and changes in the genotype will in many cases lead to different metabolite profiles. Here, a theoretical framework that may be applied to identify the function of orphan genes is presented. The approach is based on a combination of metabolome analysis combined with in silico pathway analysis. Pathway analysis may be carried out using convex analysis and a change in the active pathway structure of deletion mutants expressed in a different metabolite profile may disclose the function or the functional class of an orphan gene. The concept is illustrated using a simplified model for growth of Saccharomyces cerevisiae. (C) 2002 Wiley Periodicals, Inc.
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