期刊
JOURNAL OF CELL BIOLOGY
卷 158, 期 7, 页码 1161-1170出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200202131
关键词
H1; phosphorylation; ATP remodeling; FRAP; Tetrahymena
类别
资金
- NIGMS NIH HHS [GM21793, R01 GM021793] Funding Source: Medline
In Tetrahymena cells, phosphorylation of linker histone HI regulates transcription of specific genes. Phosphorylation acts by creating a localized negative charge patch and phenocopies the loss of H1 from chromatin, suggesting that it affects transcription by regulating the dissociation of H1 from chromatin. To test this hypothesis, we used FRAP of GFP-tagged H1 to analyze the effects of mutations that either eliminate or mimic phosphorylation on the binding of H1 to chromatin both in vivo and in vitro. We demonstrate that phosphorylation can increase the rate of dissociation of H1 from chromatin, providing a mechanism by which it can affect HI function in vivo. We also demonstrate a previously undescribed ATP-dependent process that has a global effect on the dynamic binding of linker histone to chromatin.
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